[NiFe] Group 1k-hydrogenase
This entry was last updated at: Feb. 9, 2018, 6:22 a.m.
Properties
| Group | [NiFe] Group 1: Respiratory H2-uptake [NiFe] hydrogenases |
| Subgroup | [NiFe] Group 1k: Methanophenazine-reducing |
| Function | Hydrogenotrophic respiration using heterodisulfide as a terminal electron acceptor. Enzyme transfers H2-liberated electrons through cyt b and methanophenazine to heterodisulfide reductase. |
| Activity | H2-uptake (unidirectional) |
| Oxygen tolerance | O2-sensitive |
| Localisation | Membrane-bound |
Distribution
| Ecosystem distribution | Methanogenic environments such as anoxic soils, marine sediments, and gastrointestinal tracts |
| Taxonomic distribution | Exclusive to methanogens of the order Methanosarcinales |
Genetic Organisation
e.g. Methanosarcina barkeri (WP_011306827.1):
Architecture
| Structures |
|
| Subunits | 3 |
| Subunit description | VhoB (hydrogenase large subunit) VhoA (hydrogenase small subunit) VhoC (cytochrome b subunit) |
| Catalytic site | [NiFe]-centre |
| FeS clusters | Proximal: 4Cys[4Fe4S] Medial: 4Cys[4Fe4S] Distal: 3Cys1His[4Fe4S] |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_011021166.1 | Methanosarcina acetivorans | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_011021171.1 | Methanosarcina acetivorans | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_011034101.1 | Methanosarcina mazei | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_011034107.1 | Methanosarcina mazei | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_011034237.1 | Methanosarcina mazei | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_011306827.1 | Methanosarcina barkeri | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_011306833.1 | Methanosarcina barkeri | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_012036281.1 | Methanocella arvoryzae | [NiFe] Group 1k | Euryarchaeota | Methanocellales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_012901677.1 | Methanocella paludicola | [NiFe] Group 1k | Euryarchaeota | Methanocellales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_013898215.1 | Methanosalsum zhilinae | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_014405075.1 | Methanocella conradii | [NiFe] Group 1k | Euryarchaeota | Methanocellales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_023846630.1 | Methanolobus tindarius | [NiFe] Group 1k | Euryarchaeota | Methanosarcinales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
Literature
Genetics:
- Deppenmeier, U., Blaut, M., Lentes, S., Herzberg, C., and Gottschalk, G. (1995) Analysis of the vhoGAC and vhtGAC operons from Methanosarcina mazei strain Gö1, both encoding a membrane-bound hydrogenase and a cytochrome b. Eur. J. Biochem. 227: 261-269.
- Deppenmeier, U. (1995) Different structure and expression of the operons encoding the membrane-bound hydrogenases from Methanosarcina mazei Gö1. Arch. Microbiol. 164: 370-376.
- Søndergaard, D., Petersen, C.N.S., and Greening, C. (2017) HydDB: a web tool for hydrogenase classification and analysis. Sci. Rep. 6: 34212.
Physiology:
- Ide, T., Baumer, S., and Deppenmeier, U. (1999) Energy conservation by the H2:heterodisulfide oxidoreductase from Methanosarcina mazei Gö1: identification of two proton-translocating segments. J. Bacteriol. 181:4076-4080.
- Kulkarni, G., Kridelbaugh, D.M., Guss, A.M., and Metcalf, W.W. (2009) Hydrogen is a preferred intermediate in the energy-conserving electron transport chain of Methanosarcina barkeri. Proc. Natl. Acad. Sci. U.S.A. 106: 15915-15920.
- Thauer, R.K., Kaster, A.K., Seedorf, H., Buckel, W., and Hedderich, R. (2008) Methanogenic archaea: ecologically relevant differences in energy conservation. Nat. Rev. Microbiol. 6: 579-591.
Biochemistry:
- Deppenmeier, U., Blaut, M., Schmidt, B., and Gottschalk, G. (1992) Purification and properties of a F420-nonreactive, membrane-bound hydrogenase from Methanosarcina strain Gö1. Arch. Microbiol. 157: 505-511.
- Heiden, S., Hedderich, R., Setzke, E., and Thauer, R.K. (1993) Purification of a cytochrome b containing H2:heterodisulfide oxidoreductase complex from membranes of Methanosarcina barkeri. Eur. J. Biochem. 213: 529-535.